Stability of endoglucanases from mesophilic fungus and thermophilic bacterium in acidified polyols
Chong, Barrie Fong, Harrison, Mark D., & O’Hara, Ian M. (2014) Stability of endoglucanases from mesophilic fungus and thermophilic bacterium in acidified polyols. Enzyme and Microbial Technology, 61-62, pp. 55-60.
Recent developments in chemical pretreatments of lignocellulosic biomass using polyols as co-solvents (e.g., glycerol and ethylene glycol) at temperatures less than 100 °C may allow the effective use of thermostable and non-thermostable cellulases in situ during the saccharification process. The potential of biomass saccharifying enzymes, endoglucanases (EG) from a thermophilic bacterium (Thermotoga maritima) and a mesophilic fungus (Trichoderma longibrachiatum), to retain their activity in aqueous buffer, acidified glycerol, and acidified ethylene glycol used as co-solvents at pretreatment temperatures at or below 100 °C were examined. The results show that despite its origin, T. longibrachiatum EG (Tl-EG) retained 75% of its activity after exposure to 100 °C for 5 min in aqueous buffer while T. maritima EG (Tm-EG) retained only 5% activity. However, at 90 °C both enzymes retained over 87% of their activity. In acidified (0.1% (w/w) H2SO4) glycerol, Tl-EG retained similar activity (80%) to that obtained in glycerol alone, while Tm-EG retained only 35%. With acidified ethylene glycol under these conditions, both Tl-EG and Tm-EG retained 36% of their activity. The results therefore show that Tl-EG is more stable in both acidified glycerol and ethylene glycol than Tm-EG. A preliminary kinetic study showed that pure glycerol improved the thermal stability of Tl-EG but destabilized Tm-EG, relative to the buffer solution. The half-lives of both Tl-EG and Tm-EG are 4.5 min in acidified glycerol, indicating that the effectiveness of these enzymes under typical pretreatment times of greater than 15 min will be considerably diminished. Attempts have been made to explain the differences in the results obtained between the two enzymes.
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|Item Type:||Journal Article|
|Keywords:||Cellulase, Stability, Pretreatment, Glycerol, Ethylene Glycol, Polyol|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Enzymes (060107)
Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000) > CROP AND PASTURE PRODUCTION (070300) > Crop and Pasture Biomass and Bioproducts (070304)
Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000) > CROP AND PASTURE PRODUCTION (070300) > Crop and Pasture Post Harvest Technologies (incl. Transportation and Storage) (070307)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > OTHER ENGINEERING (099900) > Agricultural Engineering (099901)
|Divisions:||Current > Research Centres > Centre for Tropical Crops and Biocommodities|
|Copyright Owner:||Copyright 2014 Elsevier|
|Copyright Statement:||This is the author’s version of a work that was accepted for publication in Enzyme and Microbial Technology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Enzyme and Microbial Technology, [in press] DOI: 10.1016/j.enzmictec.2014.04.015|
|Deposited On:||05 May 2014 00:58|
|Last Modified:||15 Jul 2015 04:14|
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