Revascularization and tissue regeneration of an empty root canal space is enhanced by a direct blood supply and stem cells

Srisuwan, Tanida, Tilkorn, Daniel J., Al-Benna, Sammy, Abberton, Keren, Messer, Harold H., & Thompson, Erik W. (2013) Revascularization and tissue regeneration of an empty root canal space is enhanced by a direct blood supply and stem cells. Dental Traumatology, 29(2), pp. 84-91.

View at publisher



Regenerative endodontics is an innovative treatment concept aiming to regenerate pulp, dentin and root structures. In the diseased or necrotic tooth, the limitation in vascular supply renders successful tissue regeneration/generation in a whole tooth challenging. The aim of this study is to evaluate the ability of vascularized tissue to develop within a pulpless tooth using tissue engineering techniques.

Materials and methods

A pulpless tooth chamber, filled with collagen I gel containing isolated rat dental pulp cells (DPC) and angiogenic growth factors, was placed into a hole created in the femoral cortex or into its own tooth socket, respectively. The gross, histological and biochemical characteristics of the de novo tissue were evaluated at 4 and 8weeks post-transplantation.


Tooth revascularization and tissue generation was observed only in the femur group, confirming the important role of vascular supply in tissue regeneration. The addition of cells and growth factors significantly promoted connective tissue production in the tooth chamber.


Successful revascularization and tissue regeneration in this model demonstrate the importance of a direct vascular supply and the advantages of a stem cell approach. © 2012 John Wiley & Sons A/S.

Impact and interest:

16 citations in Scopus
11 citations in Web of Science®
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 71128
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):4
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 22520279
Keywords: Bone formation, Dental pulp cells, Endodontics, Revascularization, Tissue regeneration, Tooth replantation
DOI: 10.1111/j.1600-9657.2012.01136.x
ISSN: 1600-4469
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Deposited On: 07 May 2014 03:28
Last Modified: 21 Jun 2017 05:01

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page