An adipoinductive role of inflammation in adipose tissue engineering : key factors in the early development of engineered soft tissues

Lilja, Heidi E., Morrison, Wayne A., Han, Xiao-Lian, Palmer, Jason, Taylor, Caroline, Tee, Richard, Möller, Andreas, Thompson, Erik W., & Abberton, Keren M. (2013) An adipoinductive role of inflammation in adipose tissue engineering : key factors in the early development of engineered soft tissues. Stem Cells and Development, 22(10), pp. 1602-1613.

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Abstract

Tissue engineering and cell implantation therapies are gaining popularity because of their potential to repair and regenerate tissues and organs. To investigate the role of inflammatory cytokines in new tissue development in engineered tissues, we have characterized the nature and timing of cell populations forming new adipose tissue in a mouse tissue engineering chamber (TEC) and characterized the gene and protein expression of cytokines in the newly developing tissues. EGFP-labeled bone marrow transplant mice and MacGreen mice were implanted with TEC for periods ranging from 0.5 days to 6 weeks. Tissues were collected at various time points and assessed for cytokine expression through ELISA and mRNA analysis or labeled for specific cell populations in the TEC. Macrophage-derived factors, such as monocyte chemotactic protein-1 (MCP-1), appear to induce adipogenesis by recruiting macrophages and bone marrow-derived precursor cells to the TEC at early time points, with a second wave of nonbone marrow-derived progenitors. Gene expression analysis suggests that TNFα, LCN-2, and Interleukin 1β are important in early stages of neo-adipogenesis. Increasing platelet-derived growth factor and vascular endothelial cell growth factor expression at early time points correlates with preadipocyte proliferation and induction of angiogenesis. This study provides new information about key elements that are involved in early development of new adipose tissue.

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20 citations in Scopus
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20 citations in Web of Science®

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ID Code: 71132
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):3
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 23231040
Additional URLs:
DOI: 10.1089/scd.2012.0451
ISSN: 1547-3287
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 2013 Mary Ann Liebert, Inc.
Deposited On: 07 May 2014 03:38
Last Modified: 08 May 2014 00:36

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