Survival of rat functional dental pulp cells in vascularized tissue engineering chambers

Srisuwan, T., Tilkorn, D.J., Al-Benna, S., Vashi, A., Penington, A., Messer, H.H., Abberton, K.M., & Thompson, E.W. (2012) Survival of rat functional dental pulp cells in vascularized tissue engineering chambers. Tissue and Cell, 44(2), pp. 111-121.

View at publisher


Regenerative endodontics aims to preserve, repair or regenerate the dental pulp tissue. Dental pulp stem cells, have a potential use in dental tissue generation. However, specific requirements to drive the dental tissue generation are still obscured. We established an in vivo model for studying the survival of dental pulp cells (DPC) and their potential to generate dental pulp tissue. DPC were mixed with collagen scaffold with or without slow release bone morphogenic protein 4 (BMP-4) and fibroblast growth factor 2 (FGF2). The cell suspension was transplanted into a vascularized tissue engineering chamber in the rat groin. Tissue constructs were harvested after 2, 4, 6, and 8 weeks and processed for histomorphological and immunohistochemical analysis. After 2 weeks newly formed tissue with new blood vessel formation were observed inside the chamber. DPC were found around dentin, particularly around the vascular pedicle and also close to the gelatin microspheres. Cell survival, was confirmed up to 8 weeks after transplantation. Dentin Sialophosphoprotein (DSPP) positive matrix production was detected in the chamber, indicating functionality of dental pulp progenitor cells. This study demonstrates the potential of our tissue engineering model to study rat dental pulp cells and their behavior in dental pulp regeneration, for future development of an alternative treatment using these techniques.

Impact and interest:

7 citations in Scopus
4 citations in Web of Science®
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 71139
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):2
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 22301418
Keywords: Basic fibroblast growth factors, Bone morphogenic protein-4, Dental pulp cells, Dental pulp stem cells, Dentin, Dentin Sialophosphoprotein, Odontoblast, Regenerative endodontics, Tissue engineering, Tissue regeneration, Vascularized tissue engineering chamber
DOI: 10.1016/j.tice.2011.12.003
ISSN: 0040-8166
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Deposited On: 07 May 2014 01:47
Last Modified: 09 May 2014 05:00

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page