An R2R3 MYB transcription factor determines red petal colour in an Actinidia (kiwifruit) hybrid population

Fraser, L.G., Seal, A.G., Montefiori, M., McGhie, T.K., Tsang, G.K., Datson, P.M., Hilario, E., Marsh, H.E., Dunn, J.K., Hellens, R.P., Davies, K.M., McNeilage, M.A., Silva, H.N.D., & Allan, A.C. (2013) An R2R3 MYB transcription factor determines red petal colour in an Actinidia (kiwifruit) hybrid population. BMC Genomics, 14(28).

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Abstract

Background

Red colour in kiwifruit results from the presence of anthocyanin pigments. Their expression, however, is complex, and varies among genotypes, species, tissues and environments. An understanding of the biosynthesis, physiology and genetics of the anthocyanins involved, and the control of their expression in different tissues, is required. A complex, the MBW complex, consisting of R2R3-MYB and bHLH transcription factors together with a WD-repeat protein, activates anthocyanin 3-O-galactosyltransferase (F3GT1) to produce anthocyanins. We examined the expression and genetic control of anthocyanins in flowers of Actinidia hybrid families segregating for red and white petal colour.

Results

Four inter-related backcross families between Actinidia chinensis Planch. var. chinensis and Actinidia eriantha Benth. were identified that segregated 1:1 for red or white petal colour. Flower pigments consisted of five known anthocyanins (two delphinidin-based and three cyanidin-based) and three unknowns. Intensity and hue differed in red petals from pale pink to deep magenta, and while intensity of colour increased with total concentration of anthocyanin, no association was found between any particular anthocyanin data and hue. Real time qPCR demonstrated that an R2R3 MYB, MYB110a, was expressed at significant levels in red-petalled progeny, but not in individuals with white petals.

A microsatellite marker was developed that identified alleles that segregated with red petal colour, but not with ovary, stamen filament, or fruit flesh colour in these families. The marker mapped to chromosome 10 in Actinidia.

The white petal phenotype was complemented by syringing Agrobacterium tumefaciens carrying Actinidia 35S::MYB110a into the petal tissue. Red pigments developed in white petals both with, and without, co-transformation with Actinidia bHLH partners. MYB110a was shown to directly activate Actinidia F3GT1 in transient assays.

Conclusions

The transcription factor, MYB110a, regulates anthocyanin production in petals in this hybrid population, but not in other flower tissues or mature fruit. The identification of delphinidin-based anthocyanins in these flowers provides candidates for colour enhancement in novel fruits.

Impact and interest:

22 citations in Scopus
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13 citations in Web of Science®

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ID Code: 71152
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):1
Export Date: 6 May 2014
Source: Scopus
Art. No.: 28
PubMed ID: 23324587
Additional URLs:
Keywords: Actinidia, Anthocyanin, Colour, Kiwifruit, MYB, Transcription factor
DOI: 10.1186/1471-2164-14-28
ISSN: 1471-2164
Divisions: Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 Fraser et al.; licensee BioMed Central Ltd.
Copyright Statement: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Deposited On: 07 May 2014 04:09
Last Modified: 08 May 2014 04:12

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