RNA interference silencing of CHS greatly alters the growth pattern of apple (Malus x domestica)

Dare, Andrew P. & Hellens, Roger P. (2013) RNA interference silencing of CHS greatly alters the growth pattern of apple (Malus x domestica). Plant Signaling and Behavior, 8(8), e25033.

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Abstract

Plants produce a vast array of phenolic compounds which are essential for their survival on land. One major class of polyphenols are the flavonoids and their formation is dependent on the enzyme chalcone synthase (CHS). In a recent study we silenced the CHS genes of apple (Malus × domestica Borkh.) and observed a loss of pigmentation in the fruit skin, flowers and stems. More surprisingly, highly silenced lines were significantly reduced in size, with small leaves and shortened internode lengths. Chemical analysis also revealed that the transgenic shoots contained greatly reduced concentrations of flavonoids which are known to modulate auxin flow. An auxin transport study verified this, with an increased auxin transport in the CHS-silenced lines. Overall, these findings suggest that auxin transport in apple has adapted to take place in the presence of high endogenous concentrations of flavonoids. Removal of these compounds therefore results in abnormal auxin movement and a highly disrupted growth pattern. © 2013 Landes Bioscience.

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ID Code: 71156
Item Type: Journal Article
Refereed: Yes
Additional Information: Export Date: 6 May 2014
Source: Scopus
Additional URLs:
Keywords: Auxin transport, Chalcone synthase, Domestica, Flavonoids, Malus ×, Phloridzin, RNA interference
DOI: 10.4161/psb.25033
ISSN: 1559-2316
Divisions: Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 07 May 2014 03:57
Last Modified: 08 May 2014 05:10

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