Repeated sequences as genetic markers in pooled tissue samples
Hellens, R.P., Ellis, T.H.N., Lee, D., & Turner, L. (1993) Repeated sequences as genetic markers in pooled tissue samples. Plant Molecular Biology, 22(1), pp. 153-157.
We show, using the PDR1 element of pea, that dispersed repeated sequences of moderate copy number can be used simply and efficiently to generate markers linked to a trait of interest. Inspection of hybridization patterns of repeated sequences to DNA mixtures of pooled genotypes is a sensitive way of detecting such markers. The large number of bands in tracks of digests of these mixtures allows the simultaneous sampling of loci at many places in the genome, and the many unlinked loci serve as internal controls. It is also shown that intensity ratios calculated from these band differences can be used to give a rough estimate of linkage distance.
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|Item Type:||Journal Article|
|Additional Information:||Cited By (since 1996):7
Export Date: 6 May 2014
PubMed ID: 8098963
|Keywords:||DNA mixtures, linkage, pea, Pisum sativum, repeated sequences|
|Divisions:||Current > QUT Faculties and Divisions > Science & Engineering Faculty|
|Deposited On:||09 May 2014 04:18|
|Last Modified:||09 May 2014 04:18|
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