Mini-scale method for nuclear run-on transcription assay in plants

Kanazawa, A., O'Dell, M., Hellens, R.P., Hitchin, E., & Metzlaff, M. (2000) Mini-scale method for nuclear run-on transcription assay in plants. Plant Molecular Biology Reporter, 18(4), pp. 377-383.

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Abstract

We present a mini-scale method for nuclear run-on transcription assay. In our method, all the centrifuge steps can be carried out by using micro-tubes for short time (5 min each) throughout the process, including isolation of transcriptionally active nuclei and purification of labeled RNA after synthesis of RNA in isolated nuclei. The assay can be performed using a small amount of plant tissue, which enables analysis of developmental changes in transcriptional status of given genes in a single individual plant. Successful results were obtained using the tissues of flower and leaf of petunia and embryo of pea, suggesting that the method is potentially applicable to a variety of plant tissues.

Impact and interest:

19 citations in Scopus
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15 citations in Web of Science®

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ID Code: 71313
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):16
Export Date: 6 May 2014
Source: Scopus
Keywords: Mini-scale method, Nuclei isolation, Run-on assay, Transcription
DOI: 10.1007/BF02825066
ISSN: 0735-9640
Divisions: Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2000 Springer
Deposited On: 09 May 2014 04:06
Last Modified: 09 May 2014 04:06

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