Direct and quantitative detection of bacteriophage by "hearing" surface detachment using a quartz crystal microbalance
Dultsev, F.N., Speight, R.E., Fiorini, M.T., Blackburn, J.M., Abell, C., Ostanin, V.P., & Klenerman, D. (2001) Direct and quantitative detection of bacteriophage by "hearing" surface detachment using a quartz crystal microbalance. Analytical Chemistry, 73(16), pp. 3935-3939.
We show that it is possible to detect specifically adsorbed bacteriophage directly by breaking the interactions between proteins displayed on the phage coat and ligands immobilized on the surface of a quartz crystal microbalance (QCM). This is achieved through increasing the amplitude of oscillation of the QCM surface and sensitively detecting the acoustic emission produced when the bacteriophage detaches from the surface. There is no interference from nonspecifically adsorbed phage. The detection is quantitative over at least 5 orders of magnitude and is sensitive enough to detect as few as 20 phage. The method has potential as a sensitive and low-cost method for virus detection.
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|Item Type:||Journal Article|
|Additional Information:||Cited By (since 1996):43 Export Date: 6 May 2014 Source: Scopus CODEN: ANCHA PubMed ID: 11534719|
|Keywords:||Quartz crystal microbalance (QCM), Acoustic emissions, Adsorption, Chemical analysis, Proteins, Quartz, Bacteriophages, ligand, analytic method, article, bacteriophage, oscillation, protein interaction, surface property, virus detection, Surface Properties|
|Divisions:||Current > QUT Faculties and Divisions > Science & Engineering Faculty|
|Deposited On:||12 May 2014 05:04|
|Last Modified:||25 Jun 2014 23:10|
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