Identification and characterisation of F3GT1 and F3GGT1, two glycosyltransferases responsible for anthocyanin biosynthesis in red-fleshed kiwifruit (Actinidia chinensis)

Montefiori, M., Espley, R.V., Stevenson, D., Cooney, J., Datson, P.M., Saiz, A., Atkinson, R.G., Hellens, R.P., & Allan, A.C. (2011) Identification and characterisation of F3GT1 and F3GGT1, two glycosyltransferases responsible for anthocyanin biosynthesis in red-fleshed kiwifruit (Actinidia chinensis). Plant Journal, 65(1), pp. 106-118.

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Abstract

Much of the diversity of anthocyanins is due to the action of glycosyltransferases, which add sugar moieties to anthocyanidins. We identified two glycosyltransferases, F3GT1 and F3GGT1, from red-fleshed kiwifruit (Actinidia chinensis) that perform sequential glycosylation steps. Red-fleshed genotypes of kiwifruit accumulate anthocyanins mainly in the form of cyanidin 3-O-xylo-galactoside. Genes in the anthocyanin and flavonoid biosynthetic pathway were identified and shown to be expressed in fruit tissue. However, only the expression of the glycosyltransferase F3GT1 was correlated with anthocyanin accumulation in red tissues. Recombinant enzyme assays in vitro and in vivo RNA interference (RNAi) demonstrated the role of F3GT1 in the production of cyanidin 3-O-galactoside. F3GGT1 was shown to further glycosylate the sugar moiety of the anthocyanins. This second glycosylation can affect the solubility and stability of the pigments and modify their colour. We show that recombinant F3GGT1 can catalyse the addition of UDP-xylose to cyanidin 3-galactoside. While F3GGT1 is responsible for the end-product of the pathway, F3GT1 is likely to be the key enzyme regulating the accumulation of anthocyanin in red-fleshed kiwifruit varieties.

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ID Code: 71427
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):16
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 21175894
Additional URLs:
Keywords: anthocyanin, fruit development, gene expression, glycosyltransferase, pigments
DOI: 10.1111/j.1365-313X.2010.04409.x
ISSN: 0960-7412
Divisions: Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2010 The New Zealand Institute for Plant and Food Research Ltd. The Plant Journal © 2010 Blackwell Publishing Ltd
Deposited On: 12 May 2014 05:55
Last Modified: 19 May 2014 05:22

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