Characterisation of a glutathione reductase gene and its genetic locus from pea (Pisum sativum L.)

Mullineaux, P., Enard, C., Hellens, R.P., & Creissen, G. (1996) Characterisation of a glutathione reductase gene and its genetic locus from pea (Pisum sativum L.). Planta, 200(2), pp. 186-194.

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A cDNA encoding the chloroplast/mitochondrial form of glutathione reductase (GR:EC 1,6,4,2) from pea (Pisum sativum L.) was used to map a single GR locus, named GORI. In two domesticated genotypes of pea (cv, Birte and JI 399) it is likely that the GORI locus contains a single gene. However, in a semi-domesticated land race of pea sequences were detected but closely related sets of GR gene sequences were in JI 281 represent either a second intact gene or a partial or pseudogene copy. A GR gene was cloned from ev. Birte, sequenced and its structure analysed. No features of the transcription or structure of the gene suggested a mechanism for generating any more than one form of . From these data plus previously published biochemical evidence was suggested a second, distinct gene encoding for the cytosolic form of GR should be present in peas. The GORI-encoded GR mRNA can be detected in all main organs of the plant and no alternative spliced species was present which could perhaps account for the generation of multiple isoforms of GR. The mismatch between the number of charge-separable isoforms in pea and the proposed number suggests that different GR isoforms arise by some form of post-transnational modification.

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14 citations in Web of Science®

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ID Code: 71434
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):13
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 8904805
Additional URLs:
Keywords: Gene structure, Gene transcript, Genetic locus, Glutathione reductase(isoforms), Pisum
DOI: 10.1007/BF00208308
ISSN: 1432-2048
Divisions: Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 12 May 2014 06:47
Last Modified: 12 May 2014 06:47

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