The binding of nuclear factors to the as-1 element in the CaMV 35S promoter is affected by cytosine methylation in vitro
Kanazawa, A., O'Dell, M., & Hellens, R.P. (2007) The binding of nuclear factors to the as-1 element in the CaMV 35S promoter is affected by cytosine methylation in vitro. Plant Biology, 9(3), pp. 435-441.
Transcriptional gene silencing (TCS) is often associated with an increased level of cytosine methylation in the affected promoters. The effect of methylation of the cauliflower mosaic virus (CaMV) 35S promoter sequence on its binding to factors present in the nuclei was analyzed by electrophoretic mobility shift assays using extracts of petunia flowers. Specific DNA-protein interactions were detected in the region of the CaMV 35S promoter that contains the as-1 element and the region between -345 and -208. The binding of protein factor(s) to the as-1 element was influenced by cytosine methylation, whereas the binding to the region between -345 and -208 was unaffected. The results suggest that cytosine methylation of the as-1 element potentially affects the activity of the CaMV 35S promoter. © Georg Thieme Verlag KG Stuttgart.
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|Item Type:||Journal Article|
|Additional Information:||Cited By (since 1996):10
Export Date: 6 May 2014
PubMed ID: 17099844
|Keywords:||Cauliflower mosaic virus 35S promoter, Cytosine methylation, DNA-protein interaction, Electrophoretic mobility shift assay, Transcriptional gene silencing|
|Deposited On:||13 May 2014 01:00|
|Last Modified:||17 Jun 2014 00:29|
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