Environmental regulation of leaf colour in red 35S : PAP1 Arabidopsis thaliana

Rowan, Daryl D., Cao, Mingshu, Lin-Wang, Kui, Cooney, Janine M., Jensen, Dwayne J., Austin, Paul T., Hunt, Martin B., Norling, Cara, Hellens, Roger P., Schaffer, Robert J., & Allan, Andrew C. (2009) Environmental regulation of leaf colour in red 35S : PAP1 Arabidopsis thaliana. New Phytologist, 182(1), pp. 102-115.

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High-temperature, low-light (HTLL) treatment of 35S:PAP1 Arabidopsis thaliana over-expressing the PAP1 (Production of Anthocyanin Pigment 1) gene results in reversible reduction of red colouration, suggesting the action of additional anthocyanin regulators. High-performance liquid chromatography (HPLC), liquid chromatography mass spectrometry (LCMS) and Affimetrix®-based microarrays were used to measure changes in anthocyanin, flavonoids, and gene expression in response to HTLL.

HTLL treatment of control and 35S:PAP1 A. thaliana resulted in a reversible reduction in the concentrations of major anthocyanins despite ongoing over-expression of the PAP1 MYB transcription factor. Twenty-one anthocyanins including eight cis-coumaryl esters were identified by LCMS. The concentrations of nine anthocyanins were reduced and those of three were increased, consistent with a sequential process of anthocyanin degradation. Analysis of gene expression showed down-regulation of flavonol and anthocyanin biosynthesis and of transport-related genes within 24 h of HTLL treatment. No catabolic genes up-regulated by HTLL were found.

Reductions in the concentrations of anthocyanins and down-regulation of the genes of anthocyanin biosynthesis were achieved by environmental manipulation, despite ongoing over-expression of PAP1. Quantitative PCR showed reduced expression of three genes (TT8, TTG1 and EGL3) of the PAP1 transcriptional complex, and increased expression of the potential transcriptional repressors AtMYB3, AtMYB6 and AtMYBL2 coincided with HTLL-induced down-regulation of anthocyanin biosynthesis.

HTLL treatment offers a model system with which to explore anthocyanin catabolism and to discover novel genes involved in the environmental control of anthocyanins.

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97 citations in Scopus
92 citations in Web of Science®
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ID Code: 71461
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):44
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 19192188
Additional URLs:
Keywords: Anthocyanin, Arabidopsis thaliana, AtMYB75, Gene expression, Liquid chromatography mass spectrometry, PAP1, Stress response
DOI: 10.1111/j.1469-8137.2008.02737.x
ISSN: 1469-8137
Divisions: Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 13 May 2014 00:47
Last Modified: 21 Jun 2017 08:01

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