The effect of exercise on the skeletal muscle phospholipidome of rats fed a high-fat diet

Mitchell, T. W., Turner, N., Else, P. L., Hulbert, A. J., Hawley, J. A., Lee, J. S., Bruce, C. R., & Blanksby, Stephen J. (2010) The effect of exercise on the skeletal muscle phospholipidome of rats fed a high-fat diet. International Journal of Molecular Sciences, 11(10), pp. 3954-3964.

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The aim of this study was to examine the effect of endurance training on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague-Dawley rats were fed a high-fat diet (78.1% energy). The rats were randomly divided into two groups, a sedentary control group and a trained group (125 min of treadmill running at 8 m/min, 4 days/wk for 4 weeks). Forty-eight hours after their last training bout phospholipids were extracted from the red and white vastus lateralis and analyzed by electrospray-ionization mass spectrometry. Exercise training was associated with significant alterations in the relative abundance of a number of phospholipid molecular species. These changes were more prominent in red vastus lateralis than white vastus lateralis. The largest observed change was an increase of similar to 30% in the abundance of 1-palmitoyl-2-linoleoyl phosphatidylcholine ions in oxidative fibers. Reductions in the relative abundance of a number of phospholipids containing long-chain n-3 polyunsaturated fatty acids were also observed. These data suggest a possible reduction in phospholipid remodeling in the trained animals. This results in a decrease in the phospholipid n-3 to n-6 ratio that may in turn influence endurance capacity.

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ID Code: 71506
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: electrospray-ionization mass spectrometry, endurance, exercise training, fatty acids, lipidomics, acid profile, biological samples, mass-spectrometry, short-term, endurance, metabolism, performance
DOI: 10.3390/ijms11103954
ISSN: 1422-0067 (online) 1661-6596 (print)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 13 May 2014 05:42
Last Modified: 09 Jun 2014 23:23

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