Systematic differences in membrane acyl composition associated with varying body mass in mammals occur in all phospholipid classes : an analysis of kidney and brain

Nealon, J. R., Blanksby, Stephen J., Mitchell, T. W., & Else, P. L. (2008) Systematic differences in membrane acyl composition associated with varying body mass in mammals occur in all phospholipid classes : an analysis of kidney and brain. Journal of Experimental Biology, 211(19), pp. 3195-3204.

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Abstract

The acyl composition of membrane phospholipids in kidney and brain of mammals of different body mass was examined. It was hypothesized that reduction in unsaturation index (number of double bonds per 100 acyl chains) of membrane phospholipids with increasing body mass in mammals would be made-up of similar changes in acyl composition across all phospholipid classes and that phospholipid class distribution would be regulated and similar in the same tissues of the different-sized mammals. The results of this study supported both hypotheses. Differences in membrane phospholipid acyl composition (i. e. decreased omega-3 fats, increased monounsaturated fats and decreased unsaturation index with increasing body size) were not restricted to any specific phospholipid molecule or to any specific phospholipid class but were observed in all phospholipid classes. With increase in body mass of mammals both monounsaturates and use of less unsaturated polyunsaturates increases at the expense of the long-chain highly unsaturated omega-3 and omega-6 polyunsaturates, producing decreases in membrane unsaturation. The distribution of membrane phospholipid classes was essentially the same in the different-sized mammals with phosphatidylcholine (PC) and phosphatidylethanolamine (PE) together constituting similar to 91% and similar to 88% of all phospholipids in kidney and brain, respectively. The lack of sphingomyelin in the mouse tissues and higher levels in larger mammals suggests an increased presence of membrane lipid rafts in larger mammals. The results of this study support the proposal that the physical properties of membranes are likely to be involved in changing metabolic rate.

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ID Code: 71510
Item Type: Journal Article
Refereed: Yes
Additional Information: Articles free to read on journal website after 6 months
Additional URLs:
Keywords: fatty acids, lipids, lipid head group, metabolism, mass spectrometry, phospholipids, glycerophospholipids, lipid class, lipid rafts, ESI-MS, basal metabolic rate, lipidomics, pump molecular activity, lipid-composition, sodium-pump, cholesterol, dynamics, biological samples, cell-membranes, crude extracts, spectrometry, metabolism, atpase
DOI: 10.1242/jeb.019968
ISSN: 0022-0949
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2008 The Company of Biologists Limited
Deposited On: 13 May 2014 04:57
Last Modified: 06 Feb 2015 02:37

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