qRT-PCR of Small RNAs

Varkonyi-Gasic, E. & Hellens, R.P. (2010) qRT-PCR of Small RNAs. Methods in Molecular Biology, 631, pp. 109-122.

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Abstract

Plant small RNAs are a class of 19- to 25-nucleotide (nt) RNA molecules that are essential for genome stability, development and differentiation, disease, cellular communication, signaling, and adaptive responses to biotic and abiotic stress. Small RNAs comprise two major RNA classes, short interfering RNAs (siRNAs) and microRNAs (miRNAs). Efficient and reliable detection and quantification of small RNA expression has become an essential step in understanding their roles in specific cells and tissues. Here we provide protocols for the detection of miRNAs by stem-loop RT-PCR. This method enables fast and reliable miRNA expression profiling from as little as 20 pg of total RNA extracted from plant tissue and is suitable for high-throughput miRNA expression analysis. In addition, this method can be used to detect other classes of small RNAs, provided the sequence is known and their GC contents are similar to those specific for miRNAs.

Impact and interest:

17 citations in Scopus
19 citations in Web of Science®
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ID Code: 71615
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):11
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 20204872
Additional URLs:
DOI: 10.1007/978-1-60761-646-7_10
ISSN: 1940-6029
Divisions: Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 15 May 2014 03:17
Last Modified: 21 Jun 2017 09:01

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