Hormonal carcinogenesis in breast cancer : cellular and molecular studies of malignant progression
Clarke, R., Skaar, T., Baumann, K., Leonessa, F., James, M., Lippman, J., Thompson, E.W., Freter, C., & Brunner, N. (1994) Hormonal carcinogenesis in breast cancer : cellular and molecular studies of malignant progression. Breast Cancer Research and Treatment, 31(2-3), pp. 237-248.
We have established and characterized a series of variant cell lines in which to identify the critical factors associated with E2-induced malignant progression, and the acquisition to tamoxifen resistance in human breast cancer. Sublines of the hormone-dependent MCF-7 cell line (MCF7/MIII and MCF7/LCC1) form stable, invasive, estrogen independent tumors in the mammary fat pads of ovariectomized athymic nude mice. These cells retain expression of both estrogen (ER) and progesterone receptors (PGR), but retain sensitivity to each of the major structural classes of antiestrogens. The tamoxifen-resistant MCF7/LCC2 cells retain sensitivity to the inhibitory effects of the steroidal antiestrogen ICI 182780. By comparing the parental hormone-dependent and variant hormone-independent cells, we have demonstrated an altered expression of some estrogen regulated genes (PGR, pS2, cathepsin D) in the hormone-independent variants. Other genes remain normally estrogen regulated (ER, laminin receptor, EGF-receptor). These data strongly implicate the altered regulation of a specific subset or network of estrogen regulated genes in the malignant progression of human breast cancer. Some of the primary response genes in this network may exhibit dose-response and induction kinetics similar to pS2, which is constitutively upregulated in the MCF7/MIII, MCF7/LCC1 and MCF7/LCC2 cells.
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|Item Type:||Journal Article|
|Additional Information:||Cited By (since 1996):49
Export Date: 6 May 2014
PubMed ID: 7881102
|Keywords:||breast cancer, carcinogenesis, estrogen, hormone dependence, malignant progression|
|Divisions:||Current > QUT Faculties and Divisions > Faculty of Health|
|Deposited On:||16 May 2014 04:18|
|Last Modified:||16 May 2014 04:18|
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