Image-guided sampling reveals increased stroma and lower glandular complexity in mammographically dense breast tissue

Lin, S. J., Cawson, J., Hill, P., Haviv, I., Jenkins, M., Hopper, J.L., Southey, M.C., Campbell, I.G., & Thompson, E.W. (2011) Image-guided sampling reveals increased stroma and lower glandular complexity in mammographically dense breast tissue. Breast Cancer Research and Treatment, 128(2), pp. 505-516.

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Mammographic density (MD) adjusted for age and body mass index (BMI) is a strong heritable breast cancer risk factor; however, its biological basis remains elusive. Previous studies assessed MD-associated histology using random sampling approaches, despite evidence that high and low MD areas exist within a breast and are negatively correlated with respect to one another. We have used an image-guided approach to sample high and low MD tissues from within individual breasts to examine the relationship between histology and degree of MD. Image-guided sampling was performed using two different methodologies on mastectomy tissues (n = 12):

(1) sampling of high and low MD regions within a slice guided by bright (high MD) and dark (low MD) areas in a slice X-ray film;

(2) sampling of high and low MD regions within a whole breast using a stereotactically guided vacuum-assisted core biopsy technique.

Pairwise analysis accounting for potential confounders (i.e. age, BMI, menopausal status, etc.) provides appropriate power for analysis despite the small sample size. High MD tissues had higher stromal (P = 0.002) and lower fat (P = 0.002) compositions, but no evidence of difference in glandular areas (P = 0.084) compared to low MD tissues from the same breast. High MD regions had higher relative gland counts (P = 0.023), and a preponderance of Type I lobules in high MD compared to low MD regions was observed in 58% of subjects (n = 7), but did not achieve significance. These findings clarify the histologic nature of high MD tissue and support hypotheses regarding the biophysical impact of dense connective tissue on mammary malignancy. They also provide important terms of reference for ongoing analyses of the underlying genetics of MD.

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21 citations in Scopus
20 citations in Web of Science®
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ID Code: 71744
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):5
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 21258862
Additional URLs:
Keywords: Fat, Glandular complexity, Mammary gland, Mammographic density, Stroma
DOI: 10.1007/s10549-011-1346-0
ISSN: 1573-7217
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Deposited On: 16 May 2014 03:49
Last Modified: 21 Jun 2017 10:01

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