Collagen-induced activation of the Mr. 72,000 type IV collagenase in normal and malignant human fibroblastoid cells

Azzam, Hala S. & Thompson, Erik W. (1992) Collagen-induced activation of the Mr. 72,000 type IV collagenase in normal and malignant human fibroblastoid cells. Cancer Research, 52(16), pp. 4540-4544.

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Although the Mr. 72,000 type IV collagenase (matrix metalloproteinase 2) has been implicated in a variety of normal and pathogenic processes, its activation mechanism in vivo is unclear. We have found that fibroblasts from normal and neoplastic human breast, as well as the sarcomatous human Hs578T and HT1080 cell lines, activate endogenous matrix metalloprotease 2 when cultured on type I collagen gels, but not on plastic, fibronectin, collagen IV, gelatin, matrigel, or basement membrane-like HR9 cell matrix. This activation is monitored by the zymographic detection of Mr 59,000 and/or Mr 62,000 species, requires 2-3 days of culture on vitrogen to manifest, is cycloheximide inhibitable, and correlates with an arborized morphology. A similar activation pattern was seen in these cells in response to Concanavalin A but not transforming growth factor β or 12-O-tetradecanoylphorbol-13-acetate. The interstitial matrix may thus play an important role in regulating matrix degradation in vivo.

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ID Code: 71820
Item Type: Journal Article
Refereed: Yes
Additional Information: Articles free to read on journal website after 12 months Cited By (since 1996):111 Export Date: 6 May 2014 Source: Scopus PubMed ID: 1322793
Additional URLs:
ISSN: 1538-7445
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Deposited On: 20 May 2014 03:52
Last Modified: 28 Jan 2015 02:16

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