Differential regulation of growth and invasiveness of MCF-7 breast cancer cells by antiestrogens

Thompson, E.W., Reich, R., Shima, T.B., Albini, A., Graf, J., Martin, G.R., Dickson, R.B., & Lippman, M.E. (1988) Differential regulation of growth and invasiveness of MCF-7 breast cancer cells by antiestrogens. Cancer Research, 48(23), pp. 6764-6768.

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Estrogen increases the ability of the estrogen-dependent MCF-7 human breast cancer cell line to both proliferate and invade through an artificial basement membrane. In studying the response of MCF-7 cells to various antiestrogens, we found that 4-hydroxytamoxifen and tamoxifen inhibited cell proliferation but increased their invasiveness. In contrast, the structurally unrelated benzothiophene antiestrogens, LY117018 and LY156758, were potent antiproliferative agents which did not stimulate invasiveness. The differential effects of these antiestrogenic agents on invasion correlated with changes in production of collagenase IV, while no significant change was seen in the chemotactic activity of the cells. Invasiveness was increased by 17β-estradiol or 4-hydroxytamoxifen after a few hours of treatment and was rapidly lost when 17β-estradiol was withdrawn. Stimulation of invasiveness with 17β-estradiol was blocked by the antiestrogen, LY117018. Cells from the MDA-MB-231 line which lacks estrogen receptors were not affected by estrogen or antiestrogen in terms of proliferation or invasion. These studies indicate that the invasiveness of MCF-7 cells is regulated by antiestrogens through the estrogen receptor and may be mediated by collagenase IV activity. Antiestrogens which reduce both the proliferation and invasiveness of these cells may be interesting new candidates for clinical application.

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ID Code: 71835
Item Type: Journal Article
Refereed: Yes
Additional Information: Articles free to read on journal website after 12 months
Cited By (since 1996):74
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 2846159
Additional URLs:
ISSN: 0008-5472
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Deposited On: 20 May 2014 02:11
Last Modified: 28 Jan 2015 02:17

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