Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties
Rae, J.M., Ramus, S.J., Waltham, M., Armes, J.E., Campbell, I.G., Clarke, R., Barndt, R.J., Johnson, M.D., & Thompson, E.W. (2004) Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties. Clinical and Experimental Metastasis, 21(6), pp. 543-552.
Recently, the tissue origin of MDA-MB-435 cell line has been the subject of considerable debate. In this study, we set out to determine whether MDA-MB-435-DTP cells shown to express melanoma-specific genes were identical to various other MDA-MB-435 cell stocks worldwide. CGH-microarray, genetic polymorphism genotyping, microsatellite fingerprint analysis and/or chromosomal number confirmed that the MDA-MB-435 cells maintained at the Lombardi Comprehensive Cancer Center (MDA-MB-435-LCC) are almost identical to the MDA-MB-435-DTP cells, and showed a very similar profile to those obtained from the same original source (MD Anderson Cancer Center) but maintained independently (MDA-MB-435-PMCC). Gene expression profile analy-sis confirmed common expression of genes among different MDA-MB-435-LCC cell stocks, and identified some unique gene products in MDA-MB-435-PMCC cells. RT-PCR analysis confirmed the expression of the melanoma marker tyrosinase across multiple MDA-MB-435 cell stocks. Collectively, our results show that the MDA-MB-435 cells used widely have identical origins to those that exhibit a melanoma-like gene expression signature, but exhibit a small degree of genotypic and phenotypic drift.
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|Item Type:||Journal Article|
|Additional Information:||Cited By (since 1996):50
Export Date: 6 May 2014
PubMed ID: 15679052
|Keywords:||Breast cancer, Cell lines, Chromosomal number, MDA-MB-435, Melanoma, Microsatellite analysis, Tyrosinase|
|Divisions:||Current > QUT Faculties and Divisions > Faculty of Health|
|Deposited On:||22 May 2014 00:47|
|Last Modified:||22 May 2014 00:47|
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