LCC15-MB cells are MDA-MB-435 : a review of misidentified breast and prostate cell lines
Thompson, E.W., Waltham, M., Ramus, S.J., Hutchins, A.M., Armes, J.E., Campbell, I.G., Williams, E.D., Thompson, P.R., Rae, J.M., Johnson, M.D., & Clarke, R. (2004) LCC15-MB cells are MDA-MB-435 : a review of misidentified breast and prostate cell lines. Clinical and Experimental Metastasis, 21(6), pp. 535-541.
Current stocks of the LCC15-MB cell line, which we originally isolated from a human breast-bone metastasis, were found to be genetically matched to the MDA-MB-435 cell line from the Lombardi Cancer Center (MDA-MB-435-LCC) using comparative genomic hybridisation, DNA microsatellite analysis and chromosomal number. LCC15-MB stocks used for our previously published studies as well as the earliest available LCC15-MB cells also showed identity to MDA-MB-435-LCC cells. The original karyotype reported for LCC15-MB cells was considerably different to that of MDA-MB-435 cells, indicating that the original LCC15-MB cells were lost to contamination by MDA-MB-435-LCC cells. Chromosome number is the simplest test to distinguish original LCC 15-MB cells (n ∼ 75) from MDA-MB-435 (n ∼ 52). Collectively, our results prove that LCC15-MB cells currently available are MDA-MB-435 cells and we suggest their re-designation as MDA-MB-435-LCC15 cells. We also review the known misclassification of breast and prostate cancer cell lines to date and have initiated a register maintained at http://www.svi.edu.au/cell_lines_registry.doc.
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|Item Type:||Journal Article|
|Additional Information:||Cited By (since 1996):12
Export Date: 6 May 2014
PubMed ID: 15679051
|Keywords:||Breast cancer, Cell lines, Chromosomal number, LCC15-MB, MDA-MB-435, Microsatellite analysis, Misidentification|
|Divisions:||Current > QUT Faculties and Divisions > Faculty of Health|
|Deposited On:||21 May 2014 03:56|
|Last Modified:||21 May 2014 03:56|
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