Analysis of unsaturated lipids by ozone-induced dissociation

Brown, S. H. J., Mitchell, T. W., & Blanksby, Stephen J. (2011) Analysis of unsaturated lipids by ozone-induced dissociation. Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids, 1811(11), pp. 807-817.

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Recent developments in analytical technologies have driven significant advances in lipid science. The sensitivity and selectivity of modern mass spectrometers can now provide for the detection and even quantification of many hundreds of lipids in a single analysis. In parallel, increasing evidence from structural biology suggests that a detailed knowledge of lipid molecular structure including carbon-carbon double bond position, stereochemistry and acyl chain regiochemistry is required to fully appreciate the biochemical role(s) of individual lipids. Here we review the capabilities and limitations of tandem mass spectrometry to provide this level of structural specificity in the analysis of lipids present in complex biological extracts. In particular, we focus on the capabilities of a novel technology termed ozone-induced dissociation to identify the position (s) of double bonds in unsaturated lipids and discuss its possible role in efforts to develop workflows that provide for complete structure elucidation of lipids by mass spectrometry alone: so-called top-down lipidomics. This article is part of a Special Issue entitled: Lipodomics and Imaging Mass Spectrom. (C) 2011 Elsevier B.V. All rights reserved.

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ID Code: 71929
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Lipid, Lipidomics, Mass spectrometry, Ozonolysis, Ozone-induced, dissociation, Collision-induced dissociation, tandem mass-spectrometry, double-bond position, protein-kinase-c, electrospray-ionization, structural-characterization, phospholipids, glycerophospholipids, elucidation, triacylglycerols, identification
DOI: 10.1016/j.bbalip.2011.04.015
ISSN: 1388-1981 (print)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
  • ACS/LP0989883
Copyright Owner: Copyright © 2011 Elsevier B.V. All rights reserved.
Deposited On: 26 May 2014 01:23
Last Modified: 27 May 2014 03:33

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