Human lens lipids differ markedly from those of commonly used experimental animals
Deeley, J. M., Mitchell, T. W., Wei, X., Korth, J., Nealon, J. R., Blanksby, Stephen J., & Truscott, R. J. W. (2008) Human lens lipids differ markedly from those of commonly used experimental animals. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1781(6-7), pp. 288-298.
Electrospray ionisation tandem mass spectrometry has allowed the unambiguous identification and quantification of individual lens phospholipids in human and six animal models. Using this approach ca. 100 unique phospholipids have been characterised. Parallel analysis of the same lens extracts by a novel direct-insertion electron-ionization technique found the cholesterol content of human lenses to be significantly higher (ca. 6 times) than lenses from the other animals. The most abundant phospholipids in all the lenses examined were choline-containing phospholipids. In rat, mouse, sheep, cow, pig and chicken, these were present largely as phosphatidylcholines, in contrast 66% of the total phospholipid in Homo sapiens was sphingomyelin, with the most abundant being dihydrosphingomyelins, in particular SM(d18:0/16:0) and SM(d18:0/24:1). The abundant glycerophospholipids within human lenses were found to be predominantly phosphatidylethanolamines and phosphatidylserines with surprisingly high concentrations of ether-linked alkyl chains identified in both classes. This study is the first to identify the phospholipid class (head-group) and assign the constituent fatty acid(s) for each lipid molecule and to quantify individual lens phospholipids using internal standards. These data clearly indicate marked differences in the membrane lipid composition of the human lens compared to commonly used animal models and thus predict a significant variation in the membrane properties of human lens fibre cells compared to those of other animals. © 2008 Elsevier B.V. All rights reserved.
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|Item Type:||Journal Article|
|Keywords:||Cataract, Cholesterol, Electrospray ionisation, Ether lipid, Fatty acid, Mass spectrometry, Phospholipid|
|Divisions:||Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2008 Elsevier B.V. All rights reserved.|
|Deposited On:||26 May 2014 00:17|
|Last Modified:||07 Jun 2014 16:53|
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