Long-term stability of adipose tissue generated from a vascularized pedicled fat flap inside a chamber

Doldere, Juergan, Thompson, Erik W., Slavin, John L., Trost, Nicholas, Cooper-White, Justin J., Cao, Yang, O'Connor, Andrea J., Penington, Anthony, Morrison, Wayne A., & Abberton, Keren M. (2011) Long-term stability of adipose tissue generated from a vascularized pedicled fat flap inside a chamber. Plastic and Reconstructive Surgery, 127(6), pp. 2283-2292.

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Abstract

Background

Numerous studies demonstrate the generation and short-term survival of adipose tissue; however, long-term persistence remains elusive. This study evaluates long-term survival and transferability of de novo adipose constructs based on a ligated vascular pedicle and tissue engineering chamber combination.

Methods

Defined adipose tissue flaps were implanted into rats in either intact or perforated domed chambers. In half of the groups, the chambers were removed after 10 weeks and the constructs transferred on their vascular pedicle to a new site, where they were observed for a further 10 weeks. In the remaining groups, the tissue construct was observed for 20 weeks inside the chamber. Tissue volume was assessed using magnetic resonance imaging and histologic measures, and constructs were assessed for stability and necrosis. Sections were assessed histologically and for proliferation using Ki-67.

Results

At 20 weeks, volume analysis revealed an increase in adipose volume from 0.04 ± 0.001 ml at the time of insertion into the chambers to 0.27 ± 0.004 ml in the closed and 0.44 ± 0.014 ml in the perforated chambers. There was an additional increase of approximately 10 to 15 percent in tissue volume in flaps that remained in chambers for 20 weeks, whereas the volume of the transferred tissue not in chambers remained unaltered. Histomorphometric assessment of the tissues documented no signs of hypertrophy, fat necrosis, or atypical changes of the newly generated tissue.

Conclusion

This study presents a promising new method of generating significant amounts of mature, vascularized, stable, and transferable adipose tissue for permanent autologous soft-tissue replacement.

Impact and interest:

26 citations in Scopus
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22 citations in Web of Science®

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ID Code: 72119
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):8
Export Date: 6 May 2014
Source: Scopus
Additional URLs:
DOI: 10.1097/PRS.0b013e3182131c3e
ISSN: 0032-1052
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Deposited On: 26 May 2014 23:35
Last Modified: 28 May 2014 00:33

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