Long-term persistence of tissue-engineered adipose flaps in a murine model to 1 year : an update

Findlay, Michael W., Messina, Aurora, Thompson, Erik W., & Morrison, Wayne A. (2009) Long-term persistence of tissue-engineered adipose flaps in a murine model to 1 year : an update. Plastic and Reconstructive Surgery, 124(4), pp. 1077-1084.

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Abstract

BACKGROUND

Tissue engineering of patient-specific adipose tissue has the potential to revolutionize reconstructive surgery. Numerous models have been described for the production of adipose tissue with success in the short term, but little has been reported on the stability of this tissue-engineered fat beyond 4 months.

METHODS

A murine model of de novo adipogenesis producing a potentially transplantable adipose tissue flap within 4 to 6 weeks was developed in the authors' laboratory. In this study, the authors assess the ability of three-chamber (44-μl volume) configurations shown to be adipogenic in previous short-term studies (autograft, n = 8; open, n = 6; fat flap, n = 11) to maintain their tissue volume for up to 12 months in vivo, to determine the most adipogenic configuration in the long term.

RESULTS

Those chambers having the most contact with existing vascularized adipose tissue (open and fat flap groups) showed increased mean adipose tissue percentage (77 ± 5.6 percent and 81 ± 6.9 percent, respectively; p < 0.0007) and volume (12 ± 6.8 μl and 30 ± 14 μl, respectively; p < 0.025) when compared with short-term controls and greater adipose tissue volume than the autograft (sealed) chamber group (4.9 ± 5.8 μl; p = 0.0001) at 1 year. Inclusion of a vascularized fat flap within the chamber produced the best results, with new fat completely filling the chamber by 1 year.

CONCLUSIONS

These findings demonstrate that fat produced by tissue engineering is capable of maintaining its volume when the appropriate microenvironment is provided. This has important implications for the application of tissue-engineering techniques in humans.

Impact and interest:

12 citations in Scopus
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11 citations in Web of Science®

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ID Code: 72120
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):6
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 19935290
Additional URLs:
DOI: 10.1097/PRS.0b013e3181b59ff6
ISSN: 0032-1052
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Deposited On: 26 May 2014 23:28
Last Modified: 30 May 2014 05:44

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