Effect of handling and fixation processes on fluorescence spectroscopy of mouse skeletal muscles under two-photon excitation

Xu, Ming G., Williams, Elizabeth D., Thompson, Erik W., & Gu, Min (2000) Effect of handling and fixation processes on fluorescence spectroscopy of mouse skeletal muscles under two-photon excitation. Applied Optics, 39(34), pp. 6312-6317.

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Abstract

We investigated the effects of handling and fixation processes on the two-photon fluorescence spectroscopy of endogenous fluorophors in mouse skeletal muscle. The skeletal muscle was handled in one of two ways: either sectioned without storage or sectioned following storage in a freezer. The two-photon fluorescence spectra measured for different storage or fixation periods show a differential among those samples that were stored in water or were fixed either in formalin or methanol. The spectroscopic results indicate that formalin was the least disruptive fixative, having only a weak effect on the two-photon fluorescence spectroscopy of muscle tissue, whereas methanol had a significant influence on one of the autofluorescence peaks. The two handling processes yielded similar spectral information, indicating no different effects between them.

Impact and interest:

12 citations in Scopus
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9 citations in Web of Science®

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ID Code: 72142
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):9
Export Date: 6 May 2014
Source: Scopus
Additional URLs:
ISSN: 2155-3165
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 2000 Optical Society of America
Deposited On: 27 May 2014 01:49
Last Modified: 27 May 2014 01:49

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