Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro

Vashi, Aditya V., Keramidaris, Efthimia, Abberton, Keren M., Morrison, Wayne A., Wilson, Jeremy L., O'Connor, Andrea J., Cooper-White, Justin J., & Thompson, Erik W. (2008) Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro. Biomaterials, 29(5), pp. 573-579.

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Due to increasing clinical demand for adipose tissue, a suitable scaffold for engineering adipose tissue constructs is needed. In this study, we have developed a three-dimensional (3-D) culture system using bone marrow-derived mesenchymal stem cells (BM-MSC) and a Pluronic F-127 hydrogel scaffold as a step towards the in vitro tissue engineering of fat. BM-MSC were dispersed into a Pluronic F-127 hydrogel with or without type I collagen added. The adipogenic differentiation of the BM-MSC was assessed by cellular morphology and further confirmed by Oil Red O staining. The BM-MSC differentiated into adipocytes in Pluronic F-127 in the presence of adipogenic stimuli over a period of 2 weeks, with some differentiation present even in absence of such stimuli. The addition of type I collagen to the Pluronic F-127 caused the BM-MSC to aggregate into clumps, thereby generating an uneven adipogenic response, which was not desirable.

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ID Code: 72147
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):39
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 17980905
Additional URLs:
Keywords: Adipose tissue engineering, Cell culture, Copolymer, In vitro test, Lipid, Thermally responsive material
DOI: 10.1016/j.biomaterials.2007.10.017
ISSN: 0142-9612
Deposited On: 27 May 2014 01:15
Last Modified: 06 Jun 2014 03:17

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