Myogel supports the ex-vivo amplification of corneal epithelial cells
Francis, D., Abberton, K., Thompson, E.W., & Daniell, M. (2009) Myogel supports the ex-vivo amplification of corneal epithelial cells. Experimental Eye Research, 88(3), pp. 339-346.
Limbal stem cell deficiency leads to conjunctivalisation of the cornea and subsequent loss of vision. The recent development of transplantation of ex-vivo amplified corneal epithelium, derived from limbal stem cells, has shown promise in treating this challenging condition. The purpose of this research was to compare a variety of cell sheet carriers for their suitability in creating a confluent corneal epithelium from amplified limbal stem cells. Cadaveric donor limbal cells were cultured using an explant technique, free of 3T3 feeder cells, on a variety of cell sheet carriers, including denuded amniotic membrane, Matrigel, Myogel and stromal extract. Comparisons in rate of growth and degree of differentiation were made, using immunocytochemistry (CK3, CK19 and ABCG2). The most rapid growth was observed on Myogel and denuded amniotic membrane, these two cell carriers also provided the most reliable substrata for achieving confluence. The putative limbal stem cell marker, ABCG2, stained positively on cells grown over Myogel and Matrigel but not for those propagated on denuded amniotic membrane. In the clinical setting amniotic membrane has been demonstrated to provide a suitable carrier for limbal stem cells and the resultant epithelium has been shown to be successful in treating limbal stem cell deficiency. Myogel may provide an alternative cell carrier with a further reduction in risk as it is has the potential to be derived from an autologous muscle biopsy in the clinical setting.
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|Item Type:||Journal Article|
|Additional Information:||Cited By (since 1996):16
Export Date: 6 May 2014
PubMed ID: 18639548
|Keywords:||cell carrier membrane, cell culture, limbal stem cell deficiency, limbal stem cells, limbus|
|Divisions:||Current > QUT Faculties and Divisions > Faculty of Health|
|Deposited On:||28 May 2014 01:46|
|Last Modified:||29 May 2014 05:34|
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