lacZ transduced human breast cancer xenografts as an in vivo model for the study of invasion and metastasis

Brünner, Nils, Thompson, Erik W., Spang-Thomsen, Mogens, Rygaard, Jørgen, Dano, Keld, & Zwiebel, James A. (1992) lacZ transduced human breast cancer xenografts as an in vivo model for the study of invasion and metastasis. European Journal of Cancer, 28(12), pp. 1989-1995.

View at publisher


A number of human cancer cell lines have been described as being invasive and metastatic in immune incompetent animals. However, it is difficult to assess metastatic spread of a subcutaneously injected or inoculated cell line, since an exact detection of all microfoci of human tumour cells in the animals by usual histological procedures would require extensive sectioning of the whole animal. To overcome this problem, we transduced human breast cancer cells with a replication-defective Moloney murine leukaemia retroviral vector (M-MuLV) containing both neo(R) (neomycin resistance) and lacZ genes. The resulting cell lines were selected for antibiotic (G418) resistance, and cell-sorted for lacZ expression. lacZ continued to be expressed in cultured cells for at least 20 passages without further G418 selection. The lacE gene codes for β-D-galactosidase, and cells expressing this gene stain blue with the chromogenic substrate X-gal. The lacZ-expressing cells retained the pre-transduction ability to traverse Matrigel in vitro, to form subcutaneous tumours in nude mice, and to grow invasively with the formation of metastases. X-gal staining showed high specificity, staining the tumour cells but not the surrounding mouse tissue on either whole tissue blocks or histological sections. The staining procedure was highly sensitive, allowing detection of microfoci of human cancer cells, and quantitative estimation of the metastatic capacity of the cells. These results indicate that lacZ transduction of human tumour cells is a powerful means of studying human cancer cell invasion and metastases in vivo.

Impact and interest:

58 citations in Scopus
Search Google Scholar™
66 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 72515
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):44 Export Date: 6 May 2014 Source: Scopus PubMed ID: 1384607
Additional URLs:
DOI: 10.1016/0959-8049(92)90245-W
ISSN: 0959-8049
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Deposited On: 03 Jun 2014 03:02
Last Modified: 03 Jun 2014 03:02

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page