An arteriovenous loop in a protected space generates a permanent, highly vascular, tissue-engineered construct

Lokmic, Zerina, Stillaert, Filip, Morrison, Wayne A., Thompson, Erik W., & Mitchell, Geraldine M. (2007) An arteriovenous loop in a protected space generates a permanent, highly vascular, tissue-engineered construct. FASEB Journal, 21(2), pp. 511-522.

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A major obstacle to 3-dimensional tissue engineering is incorporation of a functional vascular supply to support the expanding new tissue. This is overcome in an in vivo intrinsic vascularization model where an arteriovenous loop (AVL) is placed in a noncollapsible space protected by a polycarbonate chamber. Vascular development and hypoxia were examined from 3 days to 112 days by vascular casting, morphometric, and morphological techniques to understand the model's vascular growth and remodeling parameters for tissue engineering purposes. At 3 days a fibrin exudate surrounded the AVL, providing a scaffold to migrating inflammatory, endothelial, and mesenchymal cells. Capillaries formed between 3 and 7 days. Hypoxia and cell proliferation were maximal at 7 days, followed by a peak in percent vascular volume at 10 days (23.20±3.14% compared with 3.59±2.68% at 3 days, P<0.001). Maximal apoptosis was observed at 112 days. The protected space and spontaneous microcirculatory development in this model suggest it would be applicable for in vivo tissue engineering. A temporal window in a period of intense angiogenesis at 7 to 10 days is optimal for exogenous cell seeding and survival in the chamber, potentially enabling specific tissue outcomes to be achieved.

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ID Code: 72544
Item Type: Journal Article
Refereed: Yes
Additional Information: Articles free to read on journal website after 12 months
Keywords: Angiogenesis, Chamber space, Growth and remodeling, In vivo tissue engineering, Macrovascular loop
DOI: 10.1096/fj.06-6614com
ISSN: 0892-6638
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 04 Jun 2014 01:57
Last Modified: 29 Jan 2015 04:05

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