Calcium influx inhibits MT1-MMP processing and blocks MMP-2 activation

Yu, Ming, Sato, Hiroshi, Seiki, Motoharu, Spiegel, Sarah, & Thompson, Erik W. (1997) Calcium influx inhibits MT1-MMP processing and blocks MMP-2 activation. FEBS Letters, 412(3), pp. 568-572.

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We have previously reported that concanavalin A (ConA)-induced MMP-2 activation involves both transcriptional and non-transcriptional mechanisms. Here we examined the effects of calcium influx on MT1-MMP expression and MMP-2 activation in MDA-MB-231 cells. The calcium ionophore ionomycin caused a dose-dependent inhibition of ConA-induced MMP-2 activation, but had no effect on MT1-MMP mRNA levels. However, Western analysis revealed an accumulation of pro-MT1-MMP (63 kDa), indicating that ionomycin blocked the conversion of pro-MT1-MMP protein to the active 60 kDa form. This suggests that increased calcium levels inhibit the processing of MT1-MMP. This finding may help to elucidate the mechanism(s) which regulates MT1-MMP activation.

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30 citations in Scopus
31 citations in Web of Science®
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ID Code: 72547
Item Type: Journal Article
Refereed: Yes
Keywords: Activation, Calcium, Concanavalin A, Gelatinase A, Human breast cancer, MT-MMP
DOI: 10.1016/S0014-5793(97)00849-1
ISSN: 0014-5793
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 03 Jun 2014 23:44
Last Modified: 21 Jun 2017 14:01

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