Invasive and metastatic properties of MCF-7 cells and ras(H)-transfected MCF-7 cell lines

Gelmann, Edward P., Thompson, Erik W., & Sommers, Connie L. (1992) Invasive and metastatic properties of MCF-7 cells and ras(H)-transfected MCF-7 cell lines. International Journal of Cancer, 50(4), pp. 665-669.

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In vitro invasion and in vivo metastasis assays were performed with a panel of MCF-7 cells transfected with isogenic constructs of mutated ras(H) genes. Both increased levels of ras(H) expression and ras(H) oncogene activation increased activity of derivative cell lines in in vitro invasion assays. In vivo formation of spontaneous metastases was assessed after intradermal inoculation of MCF-7 cells in the vicinity of the mammary fat pads of ovariectomized nude mice. No metastases were seen in the absence of estradiol treatment of the mice. With estradiol supplementation of the mice both the ras(H)-transfected and control transfected cell lines gave a higher incidence of metastases than parental MCF-7 cells. Prolonged treatment of mice with exogenous estradiol (60 days vs. 21 days) resulted in more frequent metastases to liver and lung at the end of the 90-day observation period. In contrast to activated ras(H)-gene enhancement of metastatic capacity of rodent fibroblast and epithelial cell lines, there was no correlation of ras(H) expression with in vivo metastatic capacity of a human mammary carcinoma cell line.

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ID Code: 72561
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):20
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 1537635
Additional URLs:
DOI: 10.1002/ijc.2910500431
ISSN: 1097-0215
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Deposited On: 04 Jun 2014 04:03
Last Modified: 04 Jun 2014 04:03

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