Laminin adhesion-selected primary human colon-cancer cells are more tumorigenic than the parenteral and nonadherent cells

Jun, S. H., Thompson, Erik W., Gottardis, M., Torri, J., Yamamura, K., Kibbey, M. C., Kim, W. H., & Kleinman, H. K. (1994) Laminin adhesion-selected primary human colon-cancer cells are more tumorigenic than the parenteral and nonadherent cells. International Journal of Oncology, 4(1), pp. 55-60.

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Laminin has been shown to promote the malignant phenotype and the level of the 32/67 Kd laminin receptor has been found to correlate with Dukes' staging of colon cancer. A biopsy of a Dukes' stage B2 human colon carcinoma formed a tumor in a nude mouse after coinjection with Matrigel. The parental tumor and the murine tumor appeared identical at the histological level. A cell line LCC-C1 was established from the murine tumor. The cell line appeared moderately differentiated although it did not produce mucin in vitro; however, the xenograft in vivo did produce low levels of mucin. Laminin adherent and non-adherent cell lines were selected. The parental and the laminin-selected cell subclones adhered equally well to plastic and to fibronectin and showed similar growth rates on plastic. When injected subcutaneously into nude mice, the laminin-adherent cells formed relatively undifferentiated tumors that were twice as large as the parental cell tumors whereas the laminin non adherent cells formed very small, but highly differentiated tumors. These data demonstrate that subpopulations of tumor cells which differ in their tumorigenic properties can be selected based on their adhesion to laminin and thus provide models for studying the mechanisms of tumor growth.

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ID Code: 72574
Item Type: Journal Article
Refereed: Yes
Keywords: basement membrane, colon cancer, laminin
DOI: 10.3892/ijo.4.1.55
ISSN: 1019-6439
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 04 Jun 2014 04:33
Last Modified: 04 Jun 2014 04:33

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