GATA3 : a promising marker for metastatic breast carcinoma in serous effusion specimens

Shield, Paul W., Papadimos, David J., & Walsh, Michael D. (2014) GATA3 : a promising marker for metastatic breast carcinoma in serous effusion specimens. Cancer Cytopathology, 122(4), pp. 307-312.

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The utility of GATA3 as a marker for metastatic breast carcinoma in serous effusion specimens was investigated.


Cell block sections from 74 serous effusion specimens (32 ascitic, 2 pericardial and 40 pleural fluids) were stained with an anti-GATA3 murine monoclonal antibody. The specimens included 62 confirmed metastatic carcinomas from breast (30), female genital tract (13), gastrointestinal tract (7), lung adenocarcinoma (9), pancreas (1), kidney (1) and bladder (1). The breast carcinoma cases included 15 ductal carcinomas, 8 lobular carcinomas and in 7 the histology sub-type was not available. Twelve cases containing florid reactive mesothelial cells were also stained. The breast carcinoma cases were also stained for mammaglobin and GCDFP-15 to compare sensitivity with GATA3.


Positive nuclear staining for GATA3 was present in 90% (27/30) of metastatic breast carcinoma specimens. All non-breast metastatic carcinomas tested were negative with the exception of the single case of metastatic urothelial carcinoma. No staining was observed in any of the benign reactive cases or in benign mesothelial cells present in the malignant cell block preparations. Two cases showed weak positivity of benign lymphoid cells. Staining results were unambiguous as all positive cases showed intense nuclear staining in >50% of tumor cells. Mammaglobin (57%; 17/30) and GCDFP-15 (33%; 10/30) were less sensitive markers of breast carcinoma. If used in a panel, mammaglobin and GCFP-15 staining would have identified only one additional case to those stained with GATA3.


GATA3 may be a useful addition to immunostaining panels for serous effusion specimens when metastatic breast carcinoma is a consideration.

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ID Code: 72593
Item Type: Journal Article
Refereed: Yes
Keywords: GATA3, serous effusions, metastatic breast carcinoma, immunohitochemistry
DOI: 10.1002/cncy.21393
ISSN: 1934-662X
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2014 2014 American Cancer Society
Deposited On: 05 Jun 2014 22:43
Last Modified: 04 May 2015 22:11

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