Host rather than graft origin of Matrigel-induced adipose tissue in the murine tissue-engineering chamber

Stillaert, Filip, Findlay, Michael, Palmer, Jason, Idrizi, Rejhan, Cheang, Shirley, Messina, Aurora, Abberton, Keren M., Morrison, Wayne A., & Thompson, Erik W. (2007) Host rather than graft origin of Matrigel-induced adipose tissue in the murine tissue-engineering chamber. Tissue Engineering, 13(9), pp. 2291-2300.

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Abstract

We have recently shown that Matrigel-filled chambers containing fibroblast growth factor-2 (FGF2) and placed around an epigastric pedicle in the mouse were highly adipogenic. Contact of this construct with pre-existing tissue or a free adipose graft was required. To further investigate the mechanisms underpinning formation of new adipose tissue, we seeded these chambers with human adipose biopsies and human adipose-derived cell populations in severe combined immunodeficient mice and assessed the origin of the resultant adipose tissue after 6 weeks using species-specific probes. The tissues were negative for human-specific vimentin labeling, suggesting that the fat originates from the murine host rather than the human graft. This was supported by the strong presence of mouse-specific Cot-1 deoxyribonucleic acid labeling, and the absence of human Cot-1 labeling in the new fat. Even chambers seeded with FGF2/Matrigel containing cultured human stromal-vascular fraction (SVF) labeled strongly only for human vimentin in cells that did not have a mature adipocyte phenotype; the newly formed fat tissue was negative for human vimentin. These findings indicate that grafts placed in the chamber have an inductive function for neo-adipogenesis, rather than supplying adipocyte-precursor cells to generate the new fat tissue, and preliminary observations implicate the SVF in producing inductive factors. This surprising finding opens the door for refinement of current adipose tissue-engineering approaches.

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ID Code: 72600
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):29
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 17638518
Additional URLs:
DOI: 10.1089/ten.2006.0382
ISSN: 1937-335X
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 2007 Mary Ann Liebert, Inc. Publishers
Deposited On: 05 Jun 2014 01:55
Last Modified: 17 Jun 2014 01:59

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