Adipose tissue engineering based on the controlled release of fibroblast growth factor-2 in a collagen matrix

Vashi, Aditya V., Abberton, Keren M., Thomas, Gregory P., Morrison, Wayne A., O'Connor, Andrea J., Cooper-White, Justin J., & Thompson, Erik W. (2006) Adipose tissue engineering based on the controlled release of fibroblast growth factor-2 in a collagen matrix. Tissue Engineering, 12(11), pp. 3035-3043.

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Adipose tissue forms when basement membrane extract (Matrigel™) and fibroblast growth factor-2 (FGF-2) are added to our mouse tissue engineering chamber model. A mouse tumor extract, Matrigel is unsuitable for human clinical application, and finding an alternative to Matrigel is essential. In this study we generated adipose tissue in the chamber model without using Matrigel by controlled release of FGF-2 in a type I collagen matrix. FGF-2 was impregnated into biodegradable gelatin microspheres for its slow release. The chambers were filled with these microspheres suspended in 60 μL collagen gel. Injection of collagen containing free FGF-2 or collagen containing gelatin microspheres with buffer alone served as controls. When chambers were harvested 6 weeks after implantation, the volume and weight of the tissue obtained were higher in the group that received collagen and FGF-2 impregnated microspheres than in controls. Histologic analysis of tissue constructs showed the formation of de novo adipose tissue accompanied by angiogenesis. In contrast, control groups did not show extensive adipose tissue formation. In conclusion, this study has shown that de novo formation of adipose tissue can be achieved through controlled release of FGF-2 in collagen type I in the absence of Matrigel.

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31 citations in Scopus
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ID Code: 72601
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):41
Export Date: 6 May 2014
Source: Scopus
PubMed ID: 17518619
Additional URLs:
DOI: 10.1089/ten.2006.12.3035
ISSN: 1937-335X
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 2006 Mary Ann Liebert, Inc. Publishers
Deposited On: 05 Jun 2014 02:01
Last Modified: 05 Jun 2014 02:01

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