The role of biological extracellular matrix scaffolds in vascularized three-dimensional tissue growth in vivo
Cronin, Kevin J., Messina, Aurora, Thompson, Erik W., Morrison, Wayne A., Stevens, Geoffrey W., & Knight, Kenneth R. (2007) The role of biological extracellular matrix scaffolds in vascularized three-dimensional tissue growth in vivo. Journal of Biomedical Materials Research. Part B: Applied Biomaterials, 82(1), pp. 122-128.
An in vivo murine vascularized chamber model has been shown to generate spontaneous angiogenesis and new tissue formation. This experiment aimed to assess the effects of common biological scaffolds on tissue growth in this model. Either laminin-1, type I collagen, fibrin glue, hyaluronan, or sea sponge was inserted into silicone chambers containing the epigastric artery and vein, one end was sealed with adipose tissue and the other with bone wax, then incubated subcutaneously. After 2, 4, or 6 weeks, tissue from chambers containing collagen I, fibrin glue, hyaluronan, or no added scaffold (control) had small amounts of vascularized connective tissue. Chambers containing sea sponge had moderate connective tissue growth together with a mild "foreign body" inflammatory response. Chambers containing laminin-1, at a concentration 10-fold lower than its concentration in Matrigel™, resulted in a moderate adipogenic response. In summary, (1) biological hydrogels are resorbed and gradually replaced by vascularized connective tissue; (2) sponge-like matrices with large pores support connective tissue growth within the pores and become encapsulated with granulation tissue; (3) laminin-containing scaffolds facilitate adipogenesis. It is concluded that the nature and chemical composition of the scaffold exerts a significant influence on the amount and type of tissue generated in this in vivo chamber model.
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|Item Type:||Journal Article|
|Keywords:||Extracellular matrix, Laminin, Sea sponge, Three-dimensional scaffolds, Tissue engineering, Vascularized grafts|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Deposited On:||06 Jun 2014 00:16|
|Last Modified:||17 Jun 2014 04:15|
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