BM18 : a novel androgen-dependent human prostate cancer xenograft model derived from a bone metastasis

McCulloch, Daniel R., Opeskin, Kenneth, Thompson, Erik W., & Williams, Elizabeth D. (2005) BM18 : a novel androgen-dependent human prostate cancer xenograft model derived from a bone metastasis. Prostate, 65(1), pp. 35-43.

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Androgen-dependent prostate cancer (PrCa) xenograft models are required to study PrCa biology in the clinically relevant in vivo environment.


Human PrCa tissue from a femoral bone metastasis biopsy (BM18) was grown and passaged subcutaneously through male severe combined immune-deficient (SCID) mice. Human mitochondria (hMt), prostate specific antigen (PSA), androgen receptor (AR), cytokeratin-18 (CK-18), pan-cytokeratin, and high molecular weight-cytokeratin (HMW-CK) were assessed using immunohistochemistry (IHC). Surgical castration was performed to examine androgen dependence. Serum was collected pre- and post-castration for monitoring of PSA levels.


BM18 stained positively for hMt, PSA, AR, CK-18, pan keratin, and negatively for HMW-CK, consistent with the staining observed in the original patient material. Androgen-deprivation induced tumor regression in 10/10 castrated male SCID mice. Serum PSA levels positively correlated with BM18 tumor size.


BM18 expresses PSA and AR, and rapidly regresses in response to androgen withdrawal. This provides a new clinically significant PrCa model for the study of androgen-dependent growth.

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ID Code: 72650
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):11 Export Date: 6 May 2014 Source: Scopus PubMed ID: 15800936
Additional URLs:
Keywords: Androgen, Androgen receptor, Androgen-dependent, Prostate cancer, Prostate specific antigen, Xenograft
DOI: 10.1002/pros.20255
ISSN: 0270-4137
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Deposited On: 06 Jun 2014 00:40
Last Modified: 19 Jun 2014 00:37

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