BM18 : a novel androgen-dependent human prostate cancer xenograft model derived from a bone metastasis
McCulloch, Daniel R., Opeskin, Kenneth, Thompson, Erik W., & Williams, Elizabeth D. (2005) BM18 : a novel androgen-dependent human prostate cancer xenograft model derived from a bone metastasis. Prostate, 65(1), pp. 35-43.
Androgen-dependent prostate cancer (PrCa) xenograft models are required to study PrCa biology in the clinically relevant in vivo environment.
Human PrCa tissue from a femoral bone metastasis biopsy (BM18) was grown and passaged subcutaneously through male severe combined immune-deficient (SCID) mice. Human mitochondria (hMt), prostate specific antigen (PSA), androgen receptor (AR), cytokeratin-18 (CK-18), pan-cytokeratin, and high molecular weight-cytokeratin (HMW-CK) were assessed using immunohistochemistry (IHC). Surgical castration was performed to examine androgen dependence. Serum was collected pre- and post-castration for monitoring of PSA levels.
BM18 stained positively for hMt, PSA, AR, CK-18, pan keratin, and negatively for HMW-CK, consistent with the staining observed in the original patient material. Androgen-deprivation induced tumor regression in 10/10 castrated male SCID mice. Serum PSA levels positively correlated with BM18 tumor size.
BM18 expresses PSA and AR, and rapidly regresses in response to androgen withdrawal. This provides a new clinically significant PrCa model for the study of androgen-dependent growth.
Impact and interest:
Citation counts are sourced monthly from and citation databases.
These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.
Citations counts from theindexing service can be viewed at the linked Google Scholar™ search.
|Item Type:||Journal Article|
|Additional Information:||Cited By (since 1996):11 Export Date: 6 May 2014 Source: Scopus PubMed ID: 15800936|
|Keywords:||Androgen, Androgen receptor, Androgen-dependent, Prostate cancer, Prostate specific antigen, Xenograft|
|Divisions:||Current > QUT Faculties and Divisions > Faculty of Health|
|Deposited On:||06 Jun 2014 00:40|
|Last Modified:||19 Jun 2014 00:37|
Repository Staff Only: item control page