Progression of human breast cancer cells from hormone-dependent to hormone-independent growth both in vitro and in vivo

Clarke, Robert, Brünner, Nils, Katzenellenbogen, Benita, Thompson, Erik W., Norman, Mary J., Koppi, Caroline, Paik, Soonmyoung, Lippman, Marc E., & Dickson, Robert B. (1989) Progression of human breast cancer cells from hormone-dependent to hormone-independent growth both in vitro and in vivo. Proceedings of the National Academy of Sciences of the United States of America, 86(10), pp. 3649-3653.

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We have isolated a series of sublines of the hormone-dependent MCF-7 human breast cancer cell line after selection both in vivo and in vitro for growth in the presence of subphysiological concentrations of estrogens. These sublines represent a model system for study of the processes leading to hormonal autonomy. The cells form growing tumors in ovariectomized athymic nude mice in the absence of estrogen supplementation but retain some responsivity to estrogen as determined by stimulation of the rate of tumor growth in vivo and by induction of progesterone receptor. An ovarian-independent but hormone-responsive phenotype may occur early in the natural progression to hormone-independent and unresponsive growth in breast cancer. We observed no change in the affinity or decrease in the level of expression of estrogen receptors and progesterone receptors among the sublines and the parental cells. Epidermal growth factor receptors are not overexpressed in ovarian-independent cells. Thus, altered hormone receptor expression may be a late event in the acquisition of a hormone-independent and unresponsive phenotype. Sublines isolated by in vivo but not in vitro selection are more invasive than the parental cells both in vivo and across an artificial basement membrane in vitro. Thus, as yet unknown tumor-host interactions may be important in the development of an invasive phenotype. Furthermore, acquisition of the ovarian-independent and invasive phenotypes can occur independently.

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ID Code: 72652
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996):84 Export Date: 6 May 2014 Source: Scopus PubMed ID: 2726742
Additional URLs:
ISSN: 0027-8424
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Deposited On: 05 Jun 2014 23:56
Last Modified: 05 Jun 2014 23:56

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