Sertoli cells as paracrine modulators of DNA synthesis in rat peritubular myoid cells in culture

Raychoudhury, S. S., Thompson, Erik W., Blackshaw, A. W., & Irving, M. G. (1993) Sertoli cells as paracrine modulators of DNA synthesis in rat peritubular myoid cells in culture. Journal of Reproduction and Fertility, 99(2), pp. 513-518.

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Abstract

To determine whether Sertoli cells influence DNA synthesis by rat peritubular myoid cells in vitro, the effects of Sertoli cells on [3H]thymidine incorporation by peritubular myoid cells in a coculture situation were examined. Incubation of testicular peritubular myoid cells with Sertoli cells in coculture induced a significant increase in [3H]thymidine incorporation by peritubular myoid cells. This indicates a cell-cell cooperation between Sertoli and peritubular myoid cells in the testis in terms of DNA synthesis. Secreted factors from Sertoli cells, as tested in a parabiotic culture situation, also increased [3H]thymidine incorporation by peritubular myoid cells. Moreover, in terms of total cellular protein, cocultures of Sertoli cells and peritubular myoid cells resulted in a significant increase when compared with the monocultures, and this coculture effect substituted for the stimulatory response of serum on peritubular myoid cell monoculture. This study investigated the cooperative role of Sertoli cells and peritubular myoid cells in paracrine regulation of testicular functions.

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6 citations in Web of Science®

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ID Code: 72702
Item Type: Journal Article
Refereed: Yes
DOI: 10.1530/jrf.0.0990513
ISSN: 00224251
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 10 Jun 2014 05:20
Last Modified: 10 Jun 2014 05:20

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