Optimising the cell source and biomaterials for the generation of vascularized adipose tissue in vivo
Knight, K.R., Findlay, M.W., Abberton, K.M., Stillaert, F., Ramakrishnan, A., Keramidaris, E., Hakelius, M., Kelly, J.L., Fitzgerald, A., Penington, A.J., Simmons, P.J., Messina, A., Cooper-White, J.J., Thompson, E.W., & Morrison, W.A. (2004) Optimising the cell source and biomaterials for the generation of vascularized adipose tissue in vivo. In Transactions : 7th World Biomaterials Congress, 17 - 21 May 2004, Sydney, NSW.
We initially described a rat chamber model with an inserted arteriovenous pedicle which spontaneously generates 3-dimensional vascularized connective tissue (Tanaka Y et al., Br J Plast Surg 2000; 53: 51-7). More recently we have developed a murine chamber model containing reconstituted basement membrane (Matrigel®) and FGF-2 that generates vascularized adipose tissue in vivo (Cronin K et al., Plast Reconstr Surg 2004; in press). We have extended this work to assess the cellular and matrix requirements for the Matrigel®- induced neo-adipogenesis. We found that chambers sealed to host fat were unable to grow new adipose tissue. In these chambers the Matrigel® became vascularized with maximal outgrowth of vessels extending to the periphery at 6 weeks. A small amount of adipose tissue was found adjacent to the vessels, most likely arising from periadventitial adipose tissue. In contrast, chambers open to interaction with endogenous adipose tissue showed abundant new fat, and partial exposure to adjacent adipose tissue clearly showed neo-adipogenesis only in this area. Addition of small amounts of free fat to the closed chamber containing Matrigel® was able to induce neo-adipogenesis. Addition of small pieces of human fat also caused neo-adipogenesis in immunocompromised (SCID) mice. Also, we found Matrigel® to induce adipogenesis of Lac-Z-tagged (Rosa-26) murine bone marrow-derived mesenchymal stem cells, and cells similar to these have been isolated from human adipose tissue. Given that Matrigel® is a mouse product and cannot be used in humans, we have started investigating alternative matrix scaffolds for adipogenesis such as the PDA-approved PLGA, collagen and purified components derived from Matrigel®, such as laminin-1. The optimal conditions for adipogenesis with these matrices are still being elucidated. In conclusion, we have demonstrated that a precursor cell source inside the chamber is essential for the generation of vascularized adipose tissue in vivo. This technique offers unique potential for the reconstruction of soft tissue defects and may enable the generation of site-specific tissue using the correct microenvironment.
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|Item Type:||Conference Paper|
|Additional Information:||Export Date: 6 May 2014
|Divisions:||Current > QUT Faculties and Divisions > Faculty of Health|
|Deposited On:||11 Jun 2014 03:36|
|Last Modified:||18 Jun 2014 00:23|
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