Nucleic acid hybridizations as radioactive tool for rapid detection of banana bunchy top virus

Allam, E.K., Dale, J.L., El-Afifi, S.I., Harding, R.M., & Sadik, A.S. (2013) Nucleic acid hybridizations as radioactive tool for rapid detection of banana bunchy top virus. Pakistan Journal of Biotechnology, 10(1), pp. 1-9.

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Abstract

Banana bunchy top disease (BBTD) caused by banana bunchy top virus (BBTV) was radioactively detected by nucleic acid hybridization techniques. Results showed that, 32P-labelled insert of pBT338 was hybridized with nucleic acid extracts from BBTV-infected plants from Egypt and Australia but not with those from CMV-infected plants from Egypt. Results revealed that BBTV was greatly detected in midrib, roots, meristem, corm, leaves and pseudostem respectively. BBTV was also detected in symptomless young plants prepared from diseased plant materials grown under tissue culture conditions but was not present in those performed from healthy plant materials. The sensitivity of dot blot and Southern blot hybridizations for the detection of BBTV was also performed for the detection of BBTV.

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ID Code: 73067
Item Type: Journal Article
Refereed: Yes
Keywords: Banana Bunchy Top Disease, Banana Bunchy Top Virus, Radioactive Detection, Southern and Dot Blot Hybridizations
ISSN: 1812-1837
Divisions: Current > Research Centres > Centre for Tropical Crops and Biocommodities
Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 25 Jun 2014 22:11
Last Modified: 26 Jun 2014 23:52

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