QUT ePrints

Gene Expression Profiling of Bone Marow Stromal Cells from Juvenile, Adult, Aged and Osteoporotic Rats: With an Emphasis on Osteoporosis

Xiao, Yin, Fu, Huihua, Prasadam, Indira, Yang, Yaw-Ching, & Hollinger, Jeffrey O. (2007) Gene Expression Profiling of Bone Marow Stromal Cells from Juvenile, Adult, Aged and Osteoporotic Rats: With an Emphasis on Osteoporosis. Bone, 40(3), pp. 700-715.

This is the latest version of this eprint.

View at publisher

Abstract

PURPOSE: Osteoporosis is a multi-factorial, age-related disease with a complex etiology and mode of regulation involving a large numbers of genes. To better understand the possible relationships among genes, we fingerprinted genes in a rat model induced by ovariectomy to determine differences among osteoporotic, non-osteoporotic, aged and juvenile rats.
METHODS: We applied genome wide cDNA microarray technology to analyze genes expressed in bone marrow mesenchymal stromal cells (BMSC) and compared non-osteoporotic adult vs osteoporotic, non-osteoporotic adult vs aged, and non-osteoporotic adult vs juvenile. Rigorous statistical analysis of functional annotation (EASE program) identified over-represented biological and molecular functions with significant group wide changes (p≤0.05). Some of the expressed genes were further confirmed by quantitative RT-PCR (reverse transcription-polymerase chain reaction) RESULTS: Differences in gene expression were observed by identifying transcripts selected by t-test that were consistently changed by a minimum of two-fold. There were 195 transcripts that showed an increased expression and 109 transcripts that showed decreased expression relative to the osteoporotic condition. Of these, 75% transcripts were unknown gene products or ESTs (expressed sequence tag). A number of genes found in the aged and juvenile groups were not present in the osteoporotic rats. Functional clustering of the genes using the EASE bioinformatics program revealed that transcripts in osteoporosis were associated with signal transduction, lipid metabolism, protein metabolism, ionic and protein transport, neuropeptide and G-protein signaling pathways. Although some of the genes have previously been shown to play a key role in osteoporosis, several genes were uniquely identified in this study and likely play a role in developing aged related osteoporosis that could have compelling implications in the development of new diagnostic strategies and therapeutics for osteoporosis.
CONCLUSIONS: These data suggest that osteoporosis is associated with changes of multiple novel gene expression and that numerous pathways could play important roles in osteoporosis pathogenesis.

Impact and interest:

17 citations in Scopus
Search Google Scholar™

Citation countsare sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

578 since deposited on 02 May 2007
78 in the past twelve months

Full-text downloadsdisplays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 7315
Item Type: Journal Article
Keywords: Osteoporosis, Bone marrow stromal cells, cDNA microarray
DOI: 10.1016/j.bone.2006.10.021
ISSN: 8756-3282
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Copyright Owner: Copyright 2007 Elsevier
Copyright Statement: Reproduced in accordance with the copyright policy of the publisher.
Deposited On: 02 May 2007
Last Modified: 29 Feb 2012 23:36

Available Versions of this Item

  • Gene Expression Profiling of Bone Marow Stromal Cells from Juvenile, Adult, Aged and Osteoporotic Rats: With an Emphasis on Osteoporosis. (deposited 02 May 2007)[Currently Displayed]

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page