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Gene Expression Profiling of Cells Involved in Periodontal Regeneration

Ivanovski, Saso, Lichanska, Agnieszka M., Aniello, Elisabetta M., & Xiao, Yin (2007) Gene Expression Profiling of Cells Involved in Periodontal Regeneration. Tissue Engineering, 13(2), pp. 393-404.

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Abstract

Understanding the molecular mechanisms involved in periodontal regeneration is important for the development of more predictable clinical techniques. This study aimed to identify these mechanisms by comparing the gene expression profiles of cells derived from regenerating defects with patient-matched periodontal ligament cells. The gene profiling was carried out using Affymetrix U133A arrays containing probes for 22000 genes. Robust differences in gene expression were obtained by identifying genes that consistently changed by a minimum of two fold. Analysis of molecular function as designated by gene ontology (GO) identified differentially regulated mechanisms including protein metabolism, tyrosine kinase activity and skeletal development. The differentially expressed genes could be broadly divided into the categories of protein biosynthesis and turnover, structural constituents of the cytoskeleton and extracellular matrix, and signal transduction. The differential expression of four genes (EGR-1, elastin, osteoprotegerin and IGFBP3) was confirmed using real time PCR. Furthermore, the expression of another two differentially expressed transcripts, decorin and biglycan, was immunohistochemically confirmed in a periodontal wound healing model and the protein expression was consistent with the pattern of gene expression. This study gives insight into the molecular processes involved in periodontal regeneration and identifies cell markers that are characteristic of the regenerating periodontal tissues.

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7 citations in Scopus
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7 citations in Web of Science®

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ID Code: 7316
Item Type: Journal Article
Additional Information: Self-archiving of the author-version is not yet supported by this publisher. For more information, please refer to the journal's website (see link) or contact the author. Author contact details: yin.xiao@qut.edu.au
DOI: 10.1089/ten.2006.0082
ISSN: 1076-3279
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Copyright Owner: Copyright 2007 Mary Ann Liebert Inc.
Deposited On: 03 May 2007
Last Modified: 29 Feb 2012 23:36

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