Brca2 is involved in meiosis in Arabidopsis thaliana as suggested by its interaction with Dmc1
Siaud, Nicholas, Dray, Eloise, Gy, Isabelle, Gerard, Emmanuelle, Takvorian, Najat, & Doutriaux, Marie‐Pascale (2004) Brca2 is involved in meiosis in Arabidopsis thaliana as suggested by its interaction with Dmc1. EMBO Journal, 23(6), pp. 1392-1401.
Two BRCA2-like sequences are present in the Arabidopsis genome. Both genes are expressed in flower buds and encode nearly identical proteins, which contain four BRC motifs. In a yeast two-hybrid assay, the Arabidopsis Brca2 proteins interact with Rad51 and Dmc1. RNAi constructs aimed at silencing the BRCA2 genes at meiosis triggered a reproducible sterility phenotype, which was associated with dramatic meiosis alterations. We obtained the same phenotype upon introduction of RNAi constructs aimed at silencing the RAD51 gene at meiosis in dmc1 mutant plants. The meiotic figures we observed strongly suggest that homologous recombination is highly disturbed in these meiotic cells, leaving aberrant recombination events to repair the meiotic double-strand breaks. The 'brca2' meiotic phenotype was eliminated in spo11 mutant plants. Our experiments point to an essential role of Brca2 at meiosis in Arabidopsis. We also propose a role for Rad51 in the dmc1 context.
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|Item Type:||Journal Article|
|Additional Information:||Articles free to read on journal website after 12 months|
|Keywords:||Amino Acid Sequence, Arabidopsis/ cytology/genetics/growth & development/ metabolism, Arabidopsis Proteins, BRCA2 Protein/chemistry/genetics/ metabolism, Cell Cycle Proteins/genetics/ metabolism, DNA, Complementary/genetics, DNA-Binding Proteins/genetics/ metabolism, Flowers/genetics/growth & development/metabolism, Gene Duplication, Genes, Duplicate/genetics, Genome, Plant, Humans, Meiosis, Molecular Sequence Data, Mutation/genetics, Phenotype, Plants, Genetically Modified, Protein Binding, RNA Interference, Rad51 Recombinase, Rec A Recombinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Two-Hybrid System Techniques|
|Subjects:||Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > MEDICAL BIOCHEMISTRY AND METABOLOMICS (110100)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > MEDICAL MICROBIOLOGY (110800)
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Deposited On:||02 Jul 2014 23:55|
|Last Modified:||25 Mar 2015 02:45|
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