Alternating current electrohydrodynamics induced nanoshearing and fluid micromixing for specific capture of cancer cells

Vaidyanathan, Ramanathan, Rauf, Sakandar, Dray, Eloise, Shiddiky, Muhammad J. A., & Trau, Matt (2014) Alternating current electrohydrodynamics induced nanoshearing and fluid micromixing for specific capture of cancer cells. Chemistry - A European Journal, 20(13), pp. 3724-3729.

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Abstract

We report a new tuneable alternating current (ac) electrohydrodynamics (ac-EHD) force referred to as “nanoshearing” which involves fluid flow generated within a few nanometers of an electrode surface. This force can be externally tuned via manipulating the applied ac-EHD field strength. The ability to manipulate ac-EHD induced forces and concomitant fluid micromixing can enhance fluid transport within the capture domain of the channel (e.g., transport of analytes and hence increase target–sensor interactions). This also provides a new capability to preferentially select strongly bound analytes over onspecifically bound cells and molecules. To demonstrate the utility and versatility of nanoshearing phenomenon to specifically capture cancer cells, we present proof-of-concept data in lysed blood using two microfluidic devices containing a long array of asymmetric planar electrode pairs. Under the optimal experimental conditions, we achieved high capture efficiency (e.g., approximately 90%; %RSD=2, n=3) with a 10-fold reduction in nonspecific dsorption of non-target cells for the detection of whole cells expressing Human Epidermal Growth Factor Receptor 2 (HER2). We believe that our ac-EHD devices and the use of tuneable nanoshearing phenomenon may find relevance in a wide variety of biological and medical applications.

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ID Code: 73273
Item Type: Journal Article
Refereed: Yes
Additional URLs:
DOI: 10.1002/chem.201304590
ISSN: 1521-3765 (online ) 0947-6539 (print)
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > MEDICAL BIOCHEMISTRY AND METABOLOMICS (110100)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > MEDICAL MICROBIOLOGY (110800)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Deposited On: 03 Jul 2014 05:11
Last Modified: 03 Jul 2014 23:07

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