Irradiation, cisplatin, and 5-azacytidine upregulate cytomegalovirus promoter in tumors and muscles: Implementation of non-invasive fluorescence imaging

Kamensek, U., Sersa, G., Vidic, S., Tevz, G., Kranjc, S., & Cemazar, M. (2011) Irradiation, cisplatin, and 5-azacytidine upregulate cytomegalovirus promoter in tumors and muscles: Implementation of non-invasive fluorescence imaging. Molecular Imaging and Biology, 13(1), pp. 43-52.

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Purpose: The cytomegalovirus (CMV) promoter is one of the most commonly used promoters for expression of transgenes in mammalian cells. The aim of our study was to evaluate the role of methylation and upregulation of the CMV promoter by irradiation and the chemotherapeutic agent cisplatin in vivo using non-invasive fluorescence in vivo imaging. Procedures: Murine fibrosarcoma LPB and mammary carcinoma TS/A cells were stably transfected with plasmids encoding CMV and p21 promoter-driven green fluorescent protein (GFP) gene. Solid TS/A tumors were induced by subcutaneous injection of fluorescent tumor cells, while leg muscles were transiently transfected with plasmid encoding GFP under the control of the CMV promoter. Cells, tumors, and legs were treated either by DNA methylation inhibitor 5-azacytidine, irradiation, or cisplatin. GFP expression was determined using a fluorescence microplate reader in vitro and by non-invasive fluorescence imaging in vivo. Results: Treatment of cells, tumors, and legs with 5-azacytidine (re)activated the CMV promoter. Furthermore, treatment with irradiation or cisplatin resulted in significant upregulation of GFP expression both in vitro and in vivo. Conclusions: Observed alterations in the activity of the CMV promoter limit the usefulness of this widely used promoter as a constitutive promoter. On the other hand, inducibility of CMV promoters can be beneficially used in gene therapy when combined with standard cancer treatment, such as radiotherapy and chemotherapy. © 2010 The Author(s).

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ID Code: 73286
Item Type: Journal Article
Refereed: Yes
Keywords: Cisplatin, CMV promoter, Demethylation, Fibrosarcoma, Fluorescence imaging, In vivo, Irradiation, Mammary carcinoma, Mice, azacitidine, green fluorescent protein, protein p21, virus vector, animal cell, animal experiment, animal model, article, breast carcinoma, controlled study, Cytomegalovirus, DNA methylation, drug activity, female, fluorescence, gene expression, genetic transfection, in vitro study, in vivo study, leg muscle, mouse, nonhuman, plasmid, priority journal, promoter region, tumor cell, upregulation, viral gene delivery system, Animals, Cell Line, Tumor, Genes, Immediate-Early, Genes, Reporter, Infrared Rays, Mice, Inbred BALB C, Mice, Inbred C57BL, Microscopy, Fluorescence, Muscles, Promoter Regions, Genetic, Up-Regulation, Mammalia, Murinae, Mus
DOI: 10.1007/s11307-010-0300-6
ISSN: 15361632 (ISSN)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 08 Jul 2014 00:40
Last Modified: 08 Jul 2014 23:01

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