Enhancing itaconic acid production by Aspergillus terreus

Tevz, G., Benčina, M., & Legiša, M. (2010) Enhancing itaconic acid production by Aspergillus terreus. Applied Microbiology and Biotechnology, 87(5), pp. 1657-1664.

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Aspergillus terreus is successfully used for industrial production of itaconic acid. The acid is formed from cis-aconitate, an intermediate of the tricarboxylic (TCA) cycle, by catalytic action of cis-aconitate decarboxylase. It could be assumed that strong anaplerotic reactions that replenish the pool of the TCA cycle intermediates would enhance the synthesis and excretion rate of itaconic acid. In the phylogenetic close relative Aspergillus niger, upregulated metabolic flux through glycolysis has been described that acted as a strong anaplerotic reaction. Deregulated glycolytic flux was caused by posttranslational modification of 6-phosphofructo-1-kinase (PFK1) that resulted in formation of a highly active, citrate inhibition-resistant shorter form of the enzyme. In order to avoid complex posttranslational modification, the native A. niger pfkA gene has been modified to encode for an active shorter PFK1 fragment. By the insertion of the modified A. niger pfkA genes into the A. terreus strain, increased specific productivities of itaconic acid and final yields were documented by transformants in respect to the parental strain. On the other hand, growth rate of all transformants remained suppressed which is due to the low initial pH value of the medium, one of the prerequisites for the accumulation of itaconic acid by A. terreus mycelium. © 2010 Springer-Verlag.

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35 citations in Web of Science®
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ID Code: 73289
Item Type: Journal Article
Refereed: Yes
Keywords: 6-Phosphofructo-1-kinase, Anaplerosis, Aspergillus terreus, Itaconic acid, Posttranslational modification, Primary metabolism, Aspergillus niger, Decarboxylases, Excretion rates, Glycolytic flux, Industrial production, Initial pH value, Metabolic flux, Niger, Parental strains, Post-translational modifications, Specific productivity, TCA cycle, Transformants, Enzyme inhibition, Metabolism, Physiology, Reaction intermediates, Synthesis (chemical), Acids, carboxylic acid, catalysis, cation, enzyme activity, ester, excretion, fungus, gene expression, inhibition, pH, phylogenetics, transformation, article, DNA modification, fungal gene, fungal strain, gene insertion, mycelium, nonhuman, pfka gene, Aconitic Acid, Aspergillus, Biotechnology, Carboxy-Lyases, Fungal Proteins, Genetic Enhancement, Mutagenesis, Insertional, Phosphofructokinase-1, Protein Engineering, Succinates
DOI: 10.1007/s00253-010-2642-z
ISSN: 01757598 (ISSN)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 08 Jul 2014 00:30
Last Modified: 21 Jun 2017 20:01

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