Plasma-induced death of HepG2 cancer cells : intracellular effects of reactive species

Yan, Xu, Xiong, Zilan, Zou, Fei, Zhao, Shasha, Lu, Xinpei, Yang, Guangxiao, He, Guangyuan, & Ostrikov, Kostya (2012) Plasma-induced death of HepG2 cancer cells : intracellular effects of reactive species. Plasma Processes and Polymers, 9(1), pp. 59-66.

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Reports show that cold atmospheric-pressure plasmas can induce death of cancer cells in several minutes. However, very little is presently known about the mechanism of the plasma-induced death of cancer cells. In this paper, an atmospheric-pressure plasma plume is used to treat HepG2 cells. The experimental results show that the plasma can effectively control the intracellular concentrations of ROS, NO and lipid peroxide. It is shown that these concentrations are directly related to the mechanism of the HepG2 death, which involves several stages. First, the plasma generates NO species, which increases the NO concentration in the extracellular medium. Second, the intracellular NO concentration is increased due to the NO diffusion from the medium. Third, an increase in the intracellular NO concentration leads to the increase of the intracellular ROS concentration. Fourth, the increased oxidative stress results in more effective lipid peroxidation and consequently, cell injury. The combined action of NO, ROS and lipid peroxide species eventually results in the HepG2 cell death. The mechanism of death of human hepatocellular carcinoma cells (HepG2) induced by atmospheric-pressure room-temperature plasma, related to the plasma-controlled intracellular concentrations of reactive oxygen species (ROS), nitric oxide (NO) and lipid peroxide is revealed. Only 34.75 s are required to reduce the number of the viable HepG2 cells by 50%.

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ID Code: 73570
Item Type: Journal Article
Refereed: Yes
Keywords: atmospheric-pressure plasmas, cancer cell treatment, cell death, intracellular effects, reactive species
DOI: 10.1002/ppap.201100031
ISSN: 1612-8850
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 09 Jul 2014 02:16
Last Modified: 10 Jul 2014 01:56

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